Download e-book for kindle: Antisense Technology: General Methods, Methods of Delivery, by Abelson J.N., Simon M.I., Phillips I.M. (eds.)

By Abelson J.N., Simon M.I., Phillips I.M. (eds.)

Antisense expertise is the power to control gene expression inside mammalian cells offering strong experimental techniques for the learn of gene functionality and gene rules. for instance, tools that inhibit gene expression allow stories which probe the conventional functionality of a selected product inside a phone. Such technique can be utilized in lots of disciplines resembling pharmacology, oncology, genetics, phone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity may be a very vital software in biomedical-oriented learn. The significantly acclaimed laboratory normal for greater than 40 years, tools in Enzymology is without doubt one of the so much hugely revered guides within the box of biochemistry. on the grounds that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless suitable at the present time - actually a vital ebook for researchers in all fields of existence sciences.

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271, 1462 (1994). 134p. Ambuhl, R. Gyurko, and M. I. Phillips, Regul. Pept. 59, 171 (1995). 135M. M. McCarthy, D. A. Nielsen, and D. Goldman, Regul. Pept. 59, 163 (1995). 136A. Osen-Sand, M. Catsicas, J. K. Staple, K. A. Jones, G. Ayala, J. Knowles, G. Grenningloh, and S. Catsicas, Nature 364, 445 (1993). 30 GENERALMETHODS [ 11 Ax receptor has been reported to reduce receptor numbers in the airway smooth muscle and to inhibit adenosine, house dust mite allergen, and histamine-induced bronchoconstriction.

A. Higgins, J. R. Perez, T. A. Coleman, K. Dorshkind, W. A. McComas, U. M. Sarmiento, C. A. Rosen, and R. Narayan, Proc. Natl. Acad. Sci. A. 9tl, 9901 (1993). 141N, Hijiya, J. Zhang, M. Z. Ratajczak, J. A. Kant, K. DeRiel, M. Herlyn, G. Zon, and A. M. Gewirtz, Proc. Natl. Acad. Sci. A. 91, 4499 (1994). [1] PROGRESSIN ANTISENSETECHNOLOGY 31 in mouse liver without effects on any other isotype. The effects lasted at least 24 hr after a dose, and a clear dose-response curve was observed with a dose of 10-15 mg/kg intraperitoneally reducing PKC-a RNA levels in liver by 50% 24 hr after a dose.

Nicklin, L. Meister, R. Ziel et. , Cancer Res. $6, 3499 (1996). 143B. P. Monia, J. F. Johnston, T. Geiger, M. Muller, and D. Fabbro, Nature Med. 2, 668 (1995). 144B. P. Monia, J. F. Johnston, H. Sasmor, and L. L. Cummins, J. Biol. Chem. 271,14533 (1996). 145M. Nesterova and Y. S. Cho-Chung, Nature Med. 1, 528 (1995). 32 GENERALMETHODS [ 1] antisense in these studiesJ 46This drug has now been approved for commercialization by the FDA. More recently, ISIS 2302, an ICAM-1 inhibitor, was reported to result in statistically significant reductions in steroid doses and prolonged remissions in a small group of steroid-dependent patients with Crohn's disease.

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